The Dendrobium UH 800 is one of the orchids flowers most in demand in the flower market today. But the supply is not enough for the demand because of the natural production of this variety that takes several years. It takes quite long for the investors to recover their investments. Due to this, several researches were conducted to improve the production rate and reduce the length of maturity of dendrobium.
Today, orchid laboratories in the Philippines use standard formulation for the embryo culture of dendrobium which takes only one year to produce plantlets. A continuing study is conducted to produce more plantlets even shorter than one year by using ovules with inorganic supplements in the culture media.
Prof. Michael T. Ibesate of the Aklan State University, College of Agriculture, Forestry and Environmental Science (ASU-CAFES), and a member of the Crop Science Society of the Philippines (CSSP), conducted a study on Inorganic Substance as Nutrient Supplements in the In Vitro Culture of Dendrobium (UH 800) Ovules. In vitro are Latin terms which mean "inside the bottle", thus it refers to the dendrobium ovules cultured "inside the bottle or inside any glass container."
Prof. Ibesate has devised a way on how to produce more plantlets for this variety in a shorter period of time. He is now studying to find means for mass propagation of dendrobium. According to Prof. Ibesate," using cultured plantlets can also produce hybrid, different variety and dwarfs. With cultured plants, a flower grower cannot expect regularity because it often produces variation."
The study involves the use of Culture Media Combinations consisting of Nutrient Medium of Knudson-C. This is composed of inorganic minerals or minerals salts and organic supplements composed of tomato puree, banana homogenate and coconut water. The ovules taken from the capsule of UH 800 is sown in the fresh solid media. There are four (4) solid media used in this study. The first, labeled as KCO-A is composed of Knudson – C, organic supplements and 19-19-19 of NPK as a complete fertilizer that contains 19% of nitrogen, 19% of phosphorous, and 19% of potassium. The second is the KCO-B containing Knudson – C, organic supplements, and 21-0-0 of N (ammonium sulfate). The third is the KCO-C made up of Knudson – C and organic supplements. The last is the KCA, Knudson – C alone.
By Analysis of Variance (ANOVA) using Least Significant Difference (LSD), the laboratory experiment showed that ovules of dendrobium sown in medium of Knudson-C added with organic supplements and 19-19-19 of NPK (KCO-A) germinated earlier, almost twenty one (21) days and obtained 96.03% germination at eighty (80) days of culture. It also gained the tallest plant height (2.40 cm per plantlet), highest fresh biomass (18.05 mg per plantlet), and greatest number of roots developed (7.30 per plantlet). Among the culture media combinations tested, all the in vitro growth vigor of dendrobium (UH 800) was significantly highest in Knudson-C incorporated with organic and inorganic supplements of 19-19-19 NPK.
The study proved that the use of inorganic substance in the In Vitro cultured dendrobium ovules is effective. However, the use of ovules for the in vitro culture rather than the usual embryo, the additional supplement provided by organic substance, and the methods used in preparing it must be considered. Although using ovules instead of embryo is more complicated and arduous since it requires human intervention and proper timing for the dendrobium to produce capsule containing ovules, the result of the study is well worth it.
The use of inorganic substance is proven effective not only in producing plantlets in a shorter period of time, but also in producing better plantlets and the possibility of more flowers and varied colors it may produce.
They found out that the Dendrobium UH 800 it does not only yield a higher number of plantlets in a shorter period of time for mass production, but the growth of the said plantlets can be controlled based on the needs and demand for it. According to Prof. Ibisate, since the development of the plantlets depends on the cultured media which nourished them, its growth rate can be restricted based on the amount of medium applied to it. It is possible for man to both preserve this variety, and to cultivate diversity. /MP mailto:madyaas_pen@yahoo.com
Today, orchid laboratories in the Philippines use standard formulation for the embryo culture of dendrobium which takes only one year to produce plantlets. A continuing study is conducted to produce more plantlets even shorter than one year by using ovules with inorganic supplements in the culture media.
Prof. Michael T. Ibesate of the Aklan State University, College of Agriculture, Forestry and Environmental Science (ASU-CAFES), and a member of the Crop Science Society of the Philippines (CSSP), conducted a study on Inorganic Substance as Nutrient Supplements in the In Vitro Culture of Dendrobium (UH 800) Ovules. In vitro are Latin terms which mean "inside the bottle", thus it refers to the dendrobium ovules cultured "inside the bottle or inside any glass container."
Prof. Ibesate has devised a way on how to produce more plantlets for this variety in a shorter period of time. He is now studying to find means for mass propagation of dendrobium. According to Prof. Ibesate," using cultured plantlets can also produce hybrid, different variety and dwarfs. With cultured plants, a flower grower cannot expect regularity because it often produces variation."
The study involves the use of Culture Media Combinations consisting of Nutrient Medium of Knudson-C. This is composed of inorganic minerals or minerals salts and organic supplements composed of tomato puree, banana homogenate and coconut water. The ovules taken from the capsule of UH 800 is sown in the fresh solid media. There are four (4) solid media used in this study. The first, labeled as KCO-A is composed of Knudson – C, organic supplements and 19-19-19 of NPK as a complete fertilizer that contains 19% of nitrogen, 19% of phosphorous, and 19% of potassium. The second is the KCO-B containing Knudson – C, organic supplements, and 21-0-0 of N (ammonium sulfate). The third is the KCO-C made up of Knudson – C and organic supplements. The last is the KCA, Knudson – C alone.
By Analysis of Variance (ANOVA) using Least Significant Difference (LSD), the laboratory experiment showed that ovules of dendrobium sown in medium of Knudson-C added with organic supplements and 19-19-19 of NPK (KCO-A) germinated earlier, almost twenty one (21) days and obtained 96.03% germination at eighty (80) days of culture. It also gained the tallest plant height (2.40 cm per plantlet), highest fresh biomass (18.05 mg per plantlet), and greatest number of roots developed (7.30 per plantlet). Among the culture media combinations tested, all the in vitro growth vigor of dendrobium (UH 800) was significantly highest in Knudson-C incorporated with organic and inorganic supplements of 19-19-19 NPK.
The study proved that the use of inorganic substance in the In Vitro cultured dendrobium ovules is effective. However, the use of ovules for the in vitro culture rather than the usual embryo, the additional supplement provided by organic substance, and the methods used in preparing it must be considered. Although using ovules instead of embryo is more complicated and arduous since it requires human intervention and proper timing for the dendrobium to produce capsule containing ovules, the result of the study is well worth it.
The use of inorganic substance is proven effective not only in producing plantlets in a shorter period of time, but also in producing better plantlets and the possibility of more flowers and varied colors it may produce.
They found out that the Dendrobium UH 800 it does not only yield a higher number of plantlets in a shorter period of time for mass production, but the growth of the said plantlets can be controlled based on the needs and demand for it. According to Prof. Ibisate, since the development of the plantlets depends on the cultured media which nourished them, its growth rate can be restricted based on the amount of medium applied to it. It is possible for man to both preserve this variety, and to cultivate diversity. /MP mailto:madyaas_pen@yahoo.com
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